|Application ||WB, E|
|Other Accession||P62257, Q32LN1, NP_874356|
|Calculated MW||20655 Da|
|Antigen Region||153-183 aa|
|Other Names||Ubiquitin-conjugating enzyme E2 H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, UBE2H|
|Target/Specificity||This UBE2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-183 amino acids from the C-terminal region of human UBE2H.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBE2H Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.|
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Provided below are standard protocols that you may find useful for product applications.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2H is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs, which indicates that this enzyme is highly conserved in eukaryotes.
Hayashida, S., et al., Genomics 66(2):221-225 (2000).
Kaiser, P., et al., J. Biol. Chem. 269(12):8797-8802 (1994).
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