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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   FGF2 Antibody   

FGF2 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FGF2 Antibody AP21239a
    Anti-FGF2 Antibodyat 1:2000 dilution + A549 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - FGF2 Antibody AP21239a
    All lanes : Anti-FGF2 Antibody at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: mouse heart lysates Lane 3: rat lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - FGF2 Antibody AP21239a
    Anti-FGF2 Antibodyat 1:2000 dilution + K562 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P09038
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 30770 Da
Additional Information
Gene ID 2247
Other Names Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2, FGF2, FGFB
Target/Specificity This FGF2 antibody is generated from a rabbit immunized with a recombinant protein.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFGF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FGF2
Synonyms FGFB
Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
Cellular Location Secreted. Nucleus. Note=Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57
Tissue Location Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue.
Research Areas
Citations (0)

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Background

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.

References

Abraham J.A.,et al.Cold Spring Harb. Symp. Quant. Biol. 51:657-668(1986).
Abraham J.A.,et al.EMBO J. 5:2523-2528(1986).
Prats H.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:1836-1840(1989).
Goshima N.,et al.Nat. Methods 5:1011-1017(2008).
Hillier L.W.,et al.Nature 434:724-731(2005).

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$ 295.00
$ 99.00
Cat# AP21239a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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