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GNAI3 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GNAI3 Antibody AP21247a
    Anti-GNAI3 Antibodyat 1:2000 dilution + HT-29 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P08754
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 40532 Da
Additional Information
Gene ID 2773
Other Names Guanine nucleotide-binding protein G(k) subunit alpha, G(i) alpha-3, GNAI3
Target/Specificity This GNAI3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 309-343 amino acids from the human region of human GNAI3.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNAI3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNAI3
Function Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).
Cellular Location Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Membrane; Lipid- anchor. Note=Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
Citations (0)

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Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor- regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.

References

Didsbury J.R.,et al.FEBS Lett. 219:259-263(1987).
Beals C.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:7886-7890(1987).
Itoh H.,et al.J. Biol. Chem. 263:6656-6664(1988).
Codina J.,et al.J. Biol. Chem. 263:6746-6750(1988).
Kim S.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:4153-4157(1988).

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$ 295.00
$ 99.00
Cat# AP21247a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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