|Application ||WB, E|
|Calculated MW||40532 Da|
|Other Names||Guanine nucleotide-binding protein G(k) subunit alpha, G(i) alpha-3, GNAI3|
|Target/Specificity||This GNAI3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 309-343 amino acids from the human region of human GNAI3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GNAI3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).|
|Cellular Location||Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Membrane; Lipid- anchor. Note=Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.|
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Provided below are standard protocols that you may find useful for product applications.
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor- regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
Didsbury J.R.,et al.FEBS Lett. 219:259-263(1987).
Beals C.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:7886-7890(1987).
Itoh H.,et al.J. Biol. Chem. 263:6656-6664(1988).
Codina J.,et al.J. Biol. Chem. 263:6746-6750(1988).
Kim S.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:4153-4157(1988).
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