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PCGF6 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PCGF6 Antibody (Center) AP21285c
    Anti-PCGF6 Antibody (Center)at 1:2000 dilution + rat testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9BYE7
Reactivity Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 39047 Da
Additional Information
Gene ID 84108
Other Names Polycomb group RING finger protein 6, Mel18 and Bmi1-like RING finger, RING finger protein 134, PCGF6, MBLR, RNF134
Target/Specificity This PCGF6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 215-249 amino acids from the Central region of human PCGF6.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCGF6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCGF6
Synonyms MBLR, RNF134
Function Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Cellular Location Nucleus
Tissue Location Ubiquitous.
Citations (0)

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Background

Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

References

Akasaka T.,et al.Genes Cells 7:835-850(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).
Lee M.G.,et al.Cell 128:877-887(2007).
Maertens G.N.,et al.PLoS ONE 4:E6380-E6380(2009).

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$ 295.00
$ 99.00
Cat# AP21285c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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