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>   home   >   Products   >   Primary Antibodies   >   (Mouse) Med15 Antibody (C-term)   

(Mouse) Med15 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - (Mouse) Med15 Antibody (C-term) AP21287b
    All lanes : Anti-Med15 Antibody (C-term) at 1:2000 dilution Lane 1: rat lung lysates Lane 2: human lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 87 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q924H2
Reactivity Human, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 87061 Da
Additional Information
Other Names Mediator of RNA polymerase II transcription subunit 15, Mediator complex subunit 15, Positive cofactor 2 glutamine/Q-rich-associated protein, PC2 glutamine/Q-rich-associated protein, mPcqap, Med15, Pcqap
Target/Specificity This Mouse Med15 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 743-779 amino acids from the C-terminal region of Mouse Med15.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Med15 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Med15
Synonyms Pcqap
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).
Cellular Location Cytoplasm. Nucleus
Citations (0)

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Background

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).

References

Berti L.,et al.Genomics 74:320-332(2001).

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$ 295.00
$ 99.00
Cat# AP21287b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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