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GNA11 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - GNA11 Antibody (Center) AP21291c
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling Pdx1 with AP21291c at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and membrane staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).
  • WB - GNA11 Antibody (Center) AP21291c
    All lanes : Anti-GNA11 Antibody (Center) at 1:2000 dilution Lane 1: human testis lysates Lane 2: Hela whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession P29992
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 42123 Da
Additional Information
Gene ID 2767
Other Names Guanine nucleotide-binding protein subunit alpha-11, G alpha-11, G-protein subunit alpha-11, Guanine nucleotide-binding protein G(y) subunit alpha, GNA11, GA11
Target/Specificity This GNA11 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 115-146 amino acids from the Central region of human GNA11.
Dilution IF~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNA11 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNA11
Synonyms GA11
Function Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C.
Cellular Location Cell membrane; Lipid-anchor. Cytoplasm. Note=In testicular cells, expressed exclusively in the cytoplasm
Tissue Location Expressed in testis.
Research Areas
Citations (0)

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Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C.

References

Jiang M.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:3907-3911(1991).
Bai X.H.,et al.Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Grimwood J.,et al.Nature 428:529-535(2004).

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$ 295.00
$ 99.00
Cat# AP21291c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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