|Application ||WB, IHC-P, E|
|Calculated MW||88207 Da|
|Antigen Region||607-637 aa|
|Other Names||Ubiquitin carboxyl-terminal hydrolase 1, Deubiquitinating enzyme 1, hUBP, Ubiquitin thioesterase 1, Ubiquitin-specific-processing protease 1, USP1|
|Target/Specificity||This USP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 607-637 amino acids from the C-terminal region of human USP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||USP1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed:15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed:16531995). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed:18082604, PubMed:26388029).|
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Provided below are standard protocols that you may find useful for product applications.
Deubiquitinating enzymes (DUBs) are capable of producing a free ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins in cells. DUBs possess conserved catalytic regions known as the 'cys domain' and the 'his domain.' There are 2 distinct families of DUBs: ubiquitin-specific processing proteases (UBPs, or USPs) and ubiquitin C-terminal hydrolases (UCHs).
Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
Fujiwara, T., et al., Genomics 54(1):155-158 (1998).
D'Andrea, A., et al., Crit. Rev. Biochem. Mol. Biol. 33(5):337-352 (1998).
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