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HERC4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HERC4 Antibody (Center) AP21313c
    Anti-HERC4 Antibody (Center)at 1:2000 dilution + human testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 119 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q5GLZ8
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 118563 Da
Additional Information
Gene ID 26091
Other Names Probable E3 ubiquitin-protein ligase HERC4, 632-, HECT domain and RCC1-like domain-containing protein 4, HERC4, KIAA1593
Target/Specificity This HERC4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 445-479 amino acids from the Central region of human HERC4.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHERC4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HERC4
Synonyms KIAA1593
Function Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
Cellular Location Cytoplasm, cytosol. Note=shows a punctate cytoplasmic distribution
Tissue Location Expressed in brain and testis and detected in heart and placenta.
Citations (0)

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Background

Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates (By similarity).

References

Hochrainer K.,et al.Genomics 85:153-164(2005).
Nagase T.,et al.DNA Res. 7:273-281(2000).
Deloukas P.,et al.Nature 429:375-381(2004).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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$ 295.00
$ 99.00
Cat# AP21313c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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