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(Mouse) Epcam Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - (Mouse) Epcam Antibody (C-term) AP21333b
    AP21333b staining (Mouse) Epcam in mouse colon sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - (Mouse) Epcam Antibody (C-term) AP21333b
    All lanes : Anti-Epcam Antibody (C-term) at 1:2000 dilution Lane 1: mouse lung lysates Lane 2: mouse kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - (Mouse) Epcam Antibody (C-term) AP21333b
    All lanes : Anti-Epcam Antibody (C-term) at 1:2000 dilution Lane 1: mouse kidney lysates Lane 2: mouse colon lysates Lane 3: MCF-7 whole cell lysates Lane 4: rat colon lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q99JW5
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 35019 Da
Additional Information
Gene ID 17075
Other Names Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, EGP314, mEGP314, Protein 289A, Tumor-associated calcium signal transducer 1, CD326, Epcam, Tacstd1
Target/Specificity This Mouse Epcam antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 217-251 amino acids from the C-terminal region of Mouse Epcam.
Dilution IHC-P~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Epcam Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Epcam
Synonyms Tacstd1
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).
Cellular Location Lateral cell membrane {ECO:0000250|UniProtKB:P16422}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P16422}. Cell junction, tight junction {ECO:0000250|UniProtKB:P16422}. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction {ECO:0000250|UniProtKB:P16422}
Research Areas
Citations (0)

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Background

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).

References

Bergsagel P.L.,et al.J. Immunol. 148:590-596(1992).
Carninci P.,et al.Science 309:1559-1563(2005).

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$ 295.00
$ 99.00
Cat# AP21333b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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