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CHSY3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CHSY3 Antibody (C-term) AP21377b
    Anti-CHSY3 Antibody (C-term)at 1:1000 dilution + human uterus lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 100 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CHSY3 Antibody (C-term) AP21377b
    Anti-CHSY3 Antibody (C-term)at 1:2000 dilution + A549 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 100 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q70JA7
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 100284 Da
Additional Information
Gene ID 337876
Other Names Chondroitin sulfate synthase 3, Carbohydrate synthase 2, Chondroitin glucuronyltransferase 3, Chondroitin synthase 2, ChSy-2, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 3, N-acetylgalactosaminyltransferase 3, CHSY3, CHSY2, CSS3
Target/Specificity This CHSY3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 751-785 amino acids from the C-terminal region of human CHSY3.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHSY3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHSY3
Synonyms CHSY2, CSS3
Function Has both beta-1,3-glucuronic acid and beta-1,4-N- acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity is much reduced compared to CHSY1.
Cellular Location Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein
Tissue Location Detected at low levels in brain, cerebral cortex, uterus and small intestine.
Research Areas
Citations (0)

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Background

Has both beta-1,3-glucuronic acid and beta-1,4-N- acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity is much reduced compared to CHSY1.

References

Yada T.,et al.J. Biol. Chem. 278:39711-39725(2003).
Kamakari S.,et al.Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP21377b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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