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(Mouse) Rybp Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - (Mouse) Rybp Antibody (Center) AP21378c
    Anti-Rybp Antibody (Center)at 1:2000 dilution + NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8CCI5
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 24777 Da
Additional Information
Gene ID 56353
Other Names RING1 and YY1-binding protein, Death effector domain-associated factor, DED-associated factor, Rybp, Dedaf
Target/Specificity This Mouse Rybp antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 131-165 amino acids from the Central region of Mouse Rybp.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Rybp Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Rybp
Synonyms Dedaf
Function Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis (By similarity).
Cellular Location Nucleus. Cytoplasm. Nucleus, nucleoplasm. Note=Primarily found in the nucleus. Detected in a punctate pattern likely to represent Polycomb group (PcG) bodies
Research Areas
Citations (0)

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Background

Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis (By similarity).

References

Carninci P.,et al.Science 309:1559-1563(2005).
Garcia E.,et al.EMBO J. 18:3404-3418(1999).
Danen-van Oorschot A.A.M.M.,et al.Cell Death Differ. 11:564-573(2004).
Pirity M.K.,et al.Mol. Cell. Biol. 25:7193-7202(2005).
Arrigoni R.,et al.FEBS Lett. 580:6233-6241(2006).

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$ 295.00
$ 99.00
Cat# AP21378c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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