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Mouse Med12 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Mouse Med12 Antibody (C-term) AP21384b
    All lanes : Anti-Med12 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: C2C12 whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 245 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - Mouse Med12 Antibody (C-term) AP21384b
    Anti-Med12 Antibody (C-term)at 1:1000 dilution + NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 245 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - Mouse Med12 Antibody (C-term) AP21384b
    Anti-Med12 Antibody (C-term)at 1:1000 dilution + mouse spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 245 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - Mouse Med12 Antibody (C-term) AP21384b
    AP21384b staining Mouse Med12 in mouse stomach sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession A2AGH6
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 244561 Da
Additional Information
Gene ID 59024
Other Names Mediator of RNA polymerase II transcription subunit 12, Mediator complex subunit 12, OPA-containing protein, Thyroid hormone receptor-associated protein complex 230 kDa component, Trap230, Trinucleotide repeat-containing gene 11 protein, Med12, Kiaa0192, Mopa, Tnrc11, Trap230
Target/Specificity This Mouse Med12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1991-2025 amino acids from the C-terminal region of Mouse Med12.
Dilution WB~~1:2000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Med12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Med12
Synonyms Kiaa0192, Mopa, Tnrc11, Trap230
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)
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Background

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).

References

Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Philibert R.A.,et al.Mol. Psychiatry 3:303-309(1998).
Okazaki N.,et al.Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
Park J.,et al.Mol. Cell 50:919-930(2013).

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$ 365.00
$ 140.00
Cat# AP21384b
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