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DYNC1H1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - DYNC1H1 Antibody (C-term) AP21385b
    All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 532 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - DYNC1H1 Antibody (C-term) AP21385b
    AP21385b staining DYNC1H1 in human testis sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - DYNC1H1 Antibody (C-term) AP21385b
    All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution Lane 1: U-87 MG whole cell lysates Lane 2: PC-3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 532 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q14204
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 532408 Da
Additional Information
Gene ID 1778
Other Names Cytoplasmic dynein 1 heavy chain 1, Cytoplasmic dynein heavy chain 1, Dynein heavy chain, cytosolic, DYNC1H1, DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325
Target/Specificity This DYNC1H1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4202-4236 amino acids from the C-terminal region of human DYNC1H1.
Dilution WB~~1:2000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDYNC1H1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DYNC1H1
Synonyms DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325
Function Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)

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Background

Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.

References

Nagase T.,et al.DNA Res. 4:141-150(1997).
Ohara O.,et al.Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
Yamakawa H.,et al.Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
Vaisberg E.A.,et al.J. Cell Biol. 133:831-842(1996).
Vaisberg E.A.,et al.J. Cell Biol. 123:849-858(1993).

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$ 295.00
$ 99.00
Cat# AP21385b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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