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NFE4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NFE4 Antibody (Center) AP21387c
    All lanes : Anti-NFE4 Antibody (Center) at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: 293T/17 whole cell lysates Lane 3: human kidney lysates Lane 4: human spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q86UQ8
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 19019 Da
Additional Information
Gene ID 58160
Other Names Transcription factor NF-E4, NFE4
Target/Specificity This NFE4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 102-136 amino acids from the Central region of human NFE4.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNFE4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NFE4
Function Functions as part of the SSP (stage selector protein) complex, a complex that contributes to the preferential expression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes with enhancer elements contained in the locus control region (LCR). The complex binds to the stage selector element (SSE) in the proximal gamma-globin promoter. In contrast, isoform 2 acts as a repressor of gamma- globin gene expression by preventing NFE2 and RNA polymerase II recruitment to the promoter.
Cellular Location Nucleus.
Tissue Location Specifically expressed in fetal liver, cord blood and bone marrow. Also expressed in the K562 and HEL cell lines, which constitutively express the fetal globin genes
Citations (0)

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Background

Functions as part of the SSP (stage selector protein) complex, a complex that contributes to the preferential expression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes with enhancer elements contained in the locus control region (LCR). The complex binds to the stage selector element (SSE) in the proximal gamma-globin promoter. In contrast, isoform 2 acts as a repressor of gamma- globin gene expression by preventing NFE2 and RNA polymerase II recruitment to the promoter.

References

Zhou W.,et al.Mol. Cell. Biol. 20:7662-7672(2000).
Hillier L.W.,et al.Nature 424:157-164(2003).
Zhao Q.,et al.Blood 107:2138-2145(2006).
Zhou W.,et al.J. Biol. Chem. 279:26227-26232(2004).
Zhao Q.,et al.J. Biol. Chem. 279:41477-41486(2004).

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$ 295.00
$ 99.00
Cat# AP21387c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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