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RFX4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RFX4 Antibody (C-term) AP21391b
    Anti-RFX4 Antibody (C-term)at 1:2000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RFX4 Antibody (C-term) AP21391b
    Anti-RFX4 Antibody (C-term)at 1:2000 dilution + human brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q33E94
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 83368 Da
Additional Information
Gene ID 5992
Other Names Transcription factor RFX4, Regulatory factor X 4, Testis development protein NYD-SP10, RFX4
Target/Specificity This RFX4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 684-720 amino acids from the C-terminal region of human RFX4.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRFX4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RFX4
Function May activate transcription by interacting directly with the X-box.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00858}
Tissue Location Isoform 4 is testis-specific. Isoform 1 is expressed in brain and gliomas. Isoform 2 and isoform 3 are testis-specific (at protein level). Isoform 1 is expressed in brain (at protein level). Isoform 3 is expressed at a higher level in adult testes and ejaculated spematozoa than in fetal testes
Research Areas
Citations (0)

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Background

May activate transcription by interacting directly with the X-box.

References

Blackshear P.J.,et al.Development 130:4539-4552(2003).
Matsushita H.,et al.Cancer Sci. 96:801-809(2005).
Huang X.,et al.J. Nanosci. Nanotechnol. 5:1236-1239(2005).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.E.,et al.Nature 440:346-351(2006).

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$ 295.00
$ 99.00
Cat# AP21391b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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