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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   PORCN Antibody (Center)   

PORCN Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PORCN Antibody (Center) AP21406c
    All lanes : Anti-PORCN Antibody (Center) at 1:2000 dilution Lane 1: mouse brain lysates Lane 2: mouse cerebellum lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H237
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 52318 Da
Additional Information
Gene ID 64840
Other Names Protein-cysteine N-palmitoyltransferase porcupine, 231-, Protein MG61, PORCN, MG61, PORC, PPN
Target/Specificity This PORCN antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 138-171 amino acids from the Central region of human PORCN.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPORCN Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PORCN (HGNC:17652)
Function Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1), to Wnt proteins. Serine palmitoleylation of WNT proteins is required for efficient binding to frizzled receptors.
Cellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9JJJ7}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9JJJ7}
Tissue Location Isoform 1 is expressed in fetal brain, brain, amygdala, caudate nucleus, cerebellum, hippocampus, pituitary, thalamus, heart, skeletal muscle and testis. Isoform 4 is expressed in amygdala, corpus callosum, hippocampus, spinal cord, kidney, liver, lung, spleen, uterus, testis. Isoform 2 and isoform 3 are expressed in substantia negra, spinal cord, heart and lung
Research Areas
Citations (0)

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protein-cysteine N-palmitoyltransferase that modulates the processing of Wnt proteins by mediating serine palmitoylation of Wnt family members.


Caricasole A.,et al.Gene 288:147-157(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ross M.T.,et al.Nature 434:325-337(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Geraghty M.T.,et al.Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP21406c
(40 western blots)
Availability: In Stock
Bulk Size
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