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USP13 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP2141a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
USP13 Antibody (N-term) - Product info | |
| Application | WB
|
| Primary Accession | Q92995 |
| Other Accession | E1BMF7, NP_003931 |
| Reactivity | Human |
| Predicted | Bovine |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 97327 Da |
USP13 Antibody (N-term) - Additional info | |
| Gene ID 8975 | |
| Other Names USP13; ISOT3; Ubiquitin carboxyl-terminal hydrolase 13; Deubiquitinating enzyme 13; Isopeptidase T-3; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13 | |
| Target/Specificity This USP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164~193 amino acids from the N-terminal region of human USP13. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions USP13 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
USP13 Antibody (N-term) - Protein Information | |
| Name USP13 | |
| Synonyms ISOT3 | |
| Function Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13 Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data | |
| Tissue Location Highly expressed in ovary and testes. | |
USP13 Antibody (N-term) - Related products
AP14518a: USP13 Antibody (N-term)
AP2141a: USP13 Antibody (N-term)
AP2141c: USP13 Antibody (Center)
BP14518a: USP13 Antibody (N-term) Blocking Peptide
USP13 Antibody (N-term) - Application data
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Detection of USP13 in HeLa cells expressing exogenous USP13 by anti-USP13 Pab (Cat. #AP2141a). (Data provided by Amy Chen, Burnham Institute for Medical Research)
USP13 Antibody (N-term) - Research Areas
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BACKGROUND
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
REFERENCES
Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003). D'Andrea, A., et al., Crit. Rev. Biochem. Mol. Biol. 33(5):337-352 (1998). Timms, K.M., et al., Gene 217 (1-2), 101-106 (1998).
