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(Mouse) Bmi1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - (Mouse) Bmi1 Antibody (Center) AP21471c
    All lanes : Anti-Bmi1 Antibody (Center) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysates Lane 2: mouse kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - (Mouse) Bmi1 Antibody (Center) AP21471c
    Anti-Bmi1 Antibody (Center)at 1:2000 dilution + mouse heart lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P25916
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 36708 Da
Additional Information
Gene ID 12151
Other Names Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, Bmi1, Bmi-1, Pcgf4
Target/Specificity This Mouse Bmi1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 109-143 amino acids from the Central region of Mouse Bmi1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Bmi1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Bmi1
Synonyms Bmi-1, Pcgf4
Function Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2 (By similarity).
Cellular Location Nucleus. Cytoplasm.
Tissue Location Detected in most organs with high expression levels in thymus, heart, brain and testis
Research Areas
Citations (0)

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Background

Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2 (By similarity).

References

Haupt Y.,et al.Cell 65:753-763(1991).
van Lohuiizen M.,et al.Cell 65:737-752(1991).
Cao R.,et al.Mol. Cell 20:845-854(2005).
Buchwald G.,et al.EMBO J. 25:2465-2474(2006).

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$ 99.00
Cat# AP21471c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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