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USP21 Antibody (N-term P31)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9UK80 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 62656 Da |
| Antigen Region | 24-53 aa |
| Gene ID | 27005 |
|---|---|
| Other Names | Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23 |
| Target/Specificity | This USP21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24~53 amino acids from the N-terminal region of human USP21. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | USP21 Antibody (N-term P31) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | USP21 {ECO:0000303|PubMed:10799498, ECO:0000312|HGNC:HGNC:12620} |
|---|---|
| Function | Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator (By similarity). Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation (By similarity). Regulates gene expression via histone H2A deubiquitination (By similarity). Deubiquitinates BAZ2A/TIP5 leading to its stabilization (PubMed:26100909). Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates (PubMed:10799498). Also acts as a negative regulator of the ribosome quality control (RQC) by mediating deubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS20/uS10, thereby antagonizing ZNF598-mediated 40S ubiquitination (PubMed:32011234). |
| Cellular Location | Cytoplasm. Nucleus |
| Tissue Location | Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung. |
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Provided below are standard protocols that you may find useful for product applications.
Background
USP21 is a ubiquitin-specific protease, an enzyme that removes ubiquitin from ubiquitinated proteins. The encoded protein belongs to the C19 peptidase family, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. This protein has been reported to be capable of removing NEDD8 from NEDD8 conjugates.
References
Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
Gong, L., et al., J. Biol. Chem. 275(19):14212-14216 (2000).
Hillier, L.D., et al., Genome Res. 6(9):807-828 (1996).
Smith, T.S., et al., Biochim. Biophys. Acta 1490 (1-2), 184-188 (2000).
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