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PGM1 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - PGM1 Antibody (C-Term) AP21492b
    All lanes : Anti-PGM1 Antibody (C-Term) at 1:2000 dilution Lane 1: human liver lysate Lane 2: mouse heart lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - PGM1 Antibody (C-Term) AP21492b
    AP21492b staining PGM1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PGM1 Antibody (C-Term) AP21492b
    AP21492b staining PGM1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - PGM1 Antibody (C-Term) AP21492b
    All lanes : Anti-PGM1 Antibody (C-Term) at 1:2000 dilution Lane 1: mouse heart lysates Lane 2: Jurkat whole cell lysates Lane 3: human liver lysates Lane 4: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PGM1 Antibody (C-Term) AP21492b
    All lanes : Anti-PGM1 Antibody (C-Term) at 1:2000 dilution Lane 1: mouse brain lysates Lane 2: mouse heart lysates Lane 3: human liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P36871
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 61449 Da
Additional Information
Gene ID 5236
Other Names Phosphoglucomutase-1, PGM 1, Glucose phosphomutase 1, PGM1
Target/Specificity This PGM1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 469-501 amino acids from human PGM1.
Dilution WB~~1:2000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPGM1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PGM1
Function This enzyme participates in both the breakdown and synthesis of glucose.
Cellular Location Isoform 1: Cytoplasm.
Research Areas
Citations (0)

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Background

This enzyme participates in both the breakdown and synthesis of glucose.

References

Whitehouse D.B.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:411-415(1992).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Putt W.,et al.Biochem. J. 296:417-422(1993).

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$ 295.00
$ 99.00
Cat# AP21492b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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