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BCL9 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • IF - BCL9 Antibody (N-Term) AP21507a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AP21507a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).
  • WB - BCL9 Antibody (N-Term) AP21507a
    All lanes : Anti-BCL9 Antibody (N-Term) at 1:2000 dilution Lane 1: mouse spleen lysates Lane 2: human testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 149 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00512
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 149290 Da
Additional Information
Gene ID 607
Other Names B-cell CLL/lymphoma 9 protein, B-cell lymphoma 9 protein, Bcl-9, Protein legless homolog, BCL9
Target/Specificity This BCL9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 272-304 amino acids from human BCL9.
Dilution IF~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBCL9 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL9
Function Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity (By similarity).
Cellular Location Nucleus.
Tissue Location Detected at low levels in thymus, prostate, testis, ovary and small intestine, and at lower levels in spleen, colon and blood
Research Areas
Citations (0)

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Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity (By similarity).


Willis T.G.,et al.Blood 91:1873-1881(1998).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Kramps T.,et al.Cell 109:47-60(2002).
Matsuoka S.,et al.Science 316:1160-1166(2007).

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$ 295.00
$ 99.00
Cat# AP21507a
(40 western blots)
Availability: In Stock
Bulk Size
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