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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   USP25 Antibody (N-term)   

USP25 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - USP25 Antibody (N-term) AP2150a
    The anti-USP25 N-term Pab (Cat. #AP2150a) is used in Western blot to detect USP25 in USP25-transfected HeLa cell lysates. Transfection data is kindly provided by Dr. B. Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UHP3
Other Accession NP_037528
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 122218 Da
Antigen Region 133-163 aa
Additional Information
Gene ID 29761
Other Names Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25, USP25, USP21
Target/Specificity This USP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-163 amino acids from the N-terminal region of human USP25.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSP25 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP25
Synonyms USP21
Function Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
Cellular Location Cytoplasm
Tissue Location Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.
Research Areas
Citations (0)

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Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.


Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Groet, J., et al., Genes Chromosomes Cancer 27(2):153-161 (2000).
Valero, R., et al., Genomics 62(3):395-405 (1999).

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$ 295.00
$ 99.00
Cat# AP2150a
(40 western blots)
Availability: In Stock
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