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ADAMTS17 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ADAMTS17 Antibody (Center) AP21516c
    All lanes : Anti-ADAMTS17 Antibody (Center) at 1:500 dilution Lane 1: A431 whole cell lysate Lane 2: 293 whole cell lysate Lane 3: SK-BR-3 whole cell lysate Lane 4: RPMI 8226 whole cell lysate Lane 5: MOLT-4 whole cell lysate Lane 6: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 121 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - ADAMTS17 Antibody (Center) AP21516c
    Anti-ADAMTS17 Antibody (Center) at 1:1000 dilution + A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 121 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - ADAMTS17 Antibody (Center) AP21516c
    Immunohistochemical analysis of AP21516c on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P - ADAMTS17 Antibody (Center) AP21516c
    Immunohistochemical analysis of AP21516c on paraffin-embedded Human lung tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P - ADAMTS17 Antibody (Center) AP21516c
    Immunohistochemical analysis of AP21516c on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q8TE56
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 121127 Da
Additional Information
Gene ID 170691
Other Names A disintegrin and metalloproteinase with thrombospondin motifs 17, ADAM-TS 17, ADAM-TS17, ADAMTS-17, 3424-, ADAMTS17
Target/Specificity This ADAMTS17 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 508-540 amino acids from the Central region of human ADAMTS17.
Dilution WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADAMTS17 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADAMTS17
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Isoform 1 and isoform 2 are expressed at high levels in the lung, brain, whole eye and retina. Isoform 1 shows a weaker expression in the heart, kidney and skeletal muscle. Isoform 2 shows a weaker expression in the kidney, bone marrow and skeletal muscle. Isoform 1 and isoform 2 are expressed at high levels in the fetal heart, kidney, and whole eye, whereas a weak expression is seen in the fetal liver.
Research Areas
Citations ( 0 )

References

Cal S.,et al.Gene 283:49-62(2002).
Tan J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:671-675(2006).
Morales J.,et al.Am. J. Hum. Genet. 85:558-568(2009).

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$ 182.50
$ 70.00
Cat# AP21516c
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