|Application ||WB, E|
|Calculated MW||27335 Da|
|Other Names||Glutathione S-transferase theta-1, GST class-theta-1, Glutathione transferase T1-1, GSTT1|
|Target/Specificity||This GSTT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 216-250 amino acids from the C-terminal region of human GSTT1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GSTT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.|
|Tissue Location||Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles|
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Provided below are standard protocols that you may find useful for product applications.
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.
Pemble S.,et al.Biochem. J. 300:271-276(1994).
Jemth P.,et al.Arch. Biochem. Biophys. 348:247-254(1997).
Sprenger R.,et al.Pharmacogenetics 10:557-565(2000).
Iida A.,et al.Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
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