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USP29 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - USP29 Antibody (Center) AP2153c
    USP29 Antibody (T675) (Cat. #AP2153c) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the USP29 antibody detected the USP29 protein (arrow).
  • IHC-P - USP29 Antibody (Center) AP2153c
    USP29 Antibody (Center) (Cat. #AP2153c)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USP29 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9HBJ7
Other Accession NP_065954
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 104156 Da
Antigen Region 660-690 aa
Additional Information
Gene ID 57663
Other Names Ubiquitin carboxyl-terminal hydrolase 29, Deubiquitinating enzyme 29, Ubiquitin thioesterase 29, Ubiquitin-specific-processing protease 29, USP29
Target/Specificity This USP29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 660-690 amino acids from the Central region of human USP29.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSP29 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP29
Research Areas
Citations (0)

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Background

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

References

Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
Tureci, O., et al., Oncogene 21(24):3879-3888 (2002).
Kim, J., et al., Genome Res. 10(8):1138-1147 (2000).

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$ 295.00
$ 99.00
Cat# AP2153c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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