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RNF7 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - RNF7 Antibody (N-term) AP21564a
    AP21564a staining RNF7 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - RNF7 Antibody (N-term) AP21564a
    All lanes : Anti-RNF7 Antibody (N-term) at 1:8000 dilution Lane 1: human heart lysates Lane 2: human liver lysates Lane 3: human skeletal muscle lysates Lane 4: human testis lysates Lane 5: mouse heart lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9UBF6
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 12683 Da
Additional Information
Gene ID 9616
Other Names RING-box protein 2, Rbx2, CKII beta-binding protein 1, CKBBP1, RING finger protein 7, Regulator of cullins 2, Sensitive to apoptosis gene protein, RNF7, RBX2, ROC2, SAG
Target/Specificity This RNF7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57 amino acids from the N-terminal region of human RNF7.
Dilution IHC-P~~1:25
WB~~1:8000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF7
Synonyms RBX2, ROC2, SAG
Function Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (PubMed:10851089). CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (By similarity). Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
Cellular Location Cytoplasm. Nucleus
Tissue Location Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.
Research Areas
Citations (0)

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Background

Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.

References

Son M.-Y.,et al.Biochem. Biophys. Res. Commun. 263:743-748(1999).
Ohta T.,et al.Mol. Cell 3:535-541(1999).
Duan H.,et al.Mol. Cell. Biol. 19:3145-3155(1999).
Swaroop M.,et al.DNA Cell Biol. 20:425-434(2001).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP21564a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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