RNF7 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | Q9UBF6 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 12683 Da |
Gene ID | 9616 |
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Other Names | RING-box protein 2, Rbx2, CKII beta-binding protein 1, CKBBP1, RING finger protein 7, Regulator of cullins 2, Sensitive to apoptosis gene protein, RNF7, RBX2, ROC2, SAG |
Target/Specificity | This RNF7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57 amino acids from the N-terminal region of human RNF7. |
Dilution | WB~~1:8000 IHC-P~~1:25 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RNF7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RNF7 |
---|---|
Synonyms | RBX2, ROC2, SAG |
Function | Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (PubMed:10851089). CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (By similarity). Through the RING- type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents. |
Cellular Location | Cytoplasm. Nucleus |
Tissue Location | Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung |
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Background
Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
References
Son M.-Y.,et al.Biochem. Biophys. Res. Commun. 263:743-748(1999).
Ohta T.,et al.Mol. Cell 3:535-541(1999).
Duan H.,et al.Mol. Cell. Biol. 19:3145-3155(1999).
Swaroop M.,et al.DNA Cell Biol. 20:425-434(2001).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
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