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PSMB7 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - PSMB7 Antibody (Center) AP21570c
    AP21570c staining PSMB7 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - PSMB7 Antibody (Center) AP21570c
    All lanes : Anti-PSMB7 Antibody (Center) at 1:2000 dilution Lane 1: Raji whole cell lysates Lane 2: Hela whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PSMB7 Antibody (Center) AP21570c
    All lanes : Anti-PSMB7 Antibody (Center) at 1:8000 dilution Lane 1: Jurkat whole cell lysates Lane 2: A375 whole cell lysates Lane 3: HepG2 whole cell lysates Lane 4: MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q99436
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 29965 Da
Additional Information
Gene ID 5695
Other Names Proteasome subunit beta type-7, Macropain chain Z, Multicatalytic endopeptidase complex chain Z, Proteasome subunit Z, PSMB7, Z
Target/Specificity This PSMB7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 180-211 amino acids from the Central region of human PSMB7.
Dilution IHC-P~~1:25
WB~~1:8000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMB7 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMB7
Synonyms Z
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
Cellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00809, ECO:0000269|PubMed:18549262}. Nucleus
Tissue Location Expressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues
Research Areas
Citations ( 0 )

Background

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.

References

Hisamatsu H.,et al.J. Exp. Med. 183:1807-1816(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.

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$ 295.00
$ 99.00
Cat# AP21570c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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