Zebrafish pou5f1 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q90270 |
Reactivity | Zebrafish |
Host | Rabbit |
Clonality | polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 51505 Da |
Antigen Region | 141-177 aa |
Gene ID | 30333 |
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Other Names | POU domain, class 5, transcription factor 1, POU domain protein 2, pou5f1, gp-9, pou-2, pou2 |
Target/Specificity | This Zebrafish pou5f1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 141-177 amino acids from the Central region of Zebrafish pou5f1. |
Dilution | WB~~1:2000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Zebrafish pou5f1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | pou5f1 |
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Synonyms | gp-9, pou-2, pou2 |
Function | Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t-POU2 causes developmental retardation or arrest before gastrulation. |
Cellular Location | Nucleus. |
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Background
Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t- POU2 causes developmental retardation or arrest before gastrulation.
References
Takeda H.,et al.Genes Dev. 8:45-59(1994).
Hauptmann G.,et al.Mech. Dev. 51:127-138(1995).
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