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Zebrafish pou5f1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Zebrafish pou5f1 Antibody (Center) AP21581c
    Anti-pou5f1 Antibody (Center)at 1:2000 dilution + ZF4 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q90270
Reactivity Zebrafish
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 51505 Da
Additional Information
Gene ID 30333
Other Names POU domain, class 5, transcription factor 1, POU domain protein 2, pou5f1, gp-9, pou-2, pou2
Target/Specificity This Zebrafish pou5f1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 141-177 amino acids from the Central region of Zebrafish pou5f1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZebrafish pou5f1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name pou5f1
Synonyms gp-9, pou-2, pou2
Function Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t- POU2 causes developmental retardation or arrest before gastrulation.
Cellular Location Nucleus.
Research Areas
Citations (0)

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Background

Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t- POU2 causes developmental retardation or arrest before gastrulation.

References

Takeda H.,et al.Genes Dev. 8:45-59(1994).
Hauptmann G.,et al.Mech. Dev. 51:127-138(1995).

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$ 295.00
$ 99.00
Cat# AP21581c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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