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PSMB3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - PSMB3 Antibody (Center) AP21598c
    All lanes : Anti-PSMB3 Antibody (Center) at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: 293T/17 whole cell lysates Lane 5: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P49720
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 22949 Da
Additional Information
Gene ID 5691
Other Names Proteasome subunit beta type-3, Proteasome chain 13, Proteasome component C10-II, Proteasome theta chain, PSMB3
Target/Specificity This PSMB3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 150-185 amino acids from the Central region of human PSMB3.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMB3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMB3
Function Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Cellular Location Cytoplasm. Nucleus
Research Areas
Citations (0)

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Background

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

References

Nothwang H.G.,et al.Biochim. Biophys. Acta 1219:361-368(1994).
Bienvenut W.V.,et al.Submitted (JUL-2008) to UniProtKB.
Lubec G.,et al.Submitted (MAR-2007) to UniProtKB.
Rasmussen H.H.,et al.Electrophoresis 13:960-969(1992).
Kristensen P.,et al.Biochem. Biophys. Res. Commun. 205:1785-1789(1994).

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$ 295.00
$ 99.00
Cat# AP21598c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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