|Application ||WB, E|
|Other Accession||P42567, NP_001972|
|Calculated MW||98656 Da|
|Antigen Region||840-870 aa|
|Other Names||Epidermal growth factor receptor substrate 15, Protein Eps15, Protein AF-1p, EPS15, AF1P|
|Target/Specificity||This EPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 840-870 amino acids from the C-terminal region of human EPS15.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Precautions||EPS15 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta- 1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.|
|Cellular Location||Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit Note=Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin- rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
EPS15 is involved in cell growth regulation, possibly via the regulation of mitogenic signals and control of cell proliferation. EPS15 also participates in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Potential interaction partners include AP2A2, STN2, EPN1, and CRK via its SH3-binding sites. EPS15 is ubiquitously expressed. Phosphorylation on Tyr-849 is involved in the internalization of EGFR. EPS15 is not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis. This protein is involved in a t(1;11)(p32;q23) chromosomal translocation in acute leukemias causing fusion to the trithorax (MLL or HRX) gene product which contains DNA-binding motifs resulting in a rogue activator protein. Structurally, EPS15 contains 2 EF-hand calcium-binding domains, 3 EH domains, and 2 ubiquitin-interacting motif (UIM) repeats.
de Beer, T., et al., Nat. Struct. Biol. 7(11):1018-1022 (2000).
Enmon, J.L., et al., Biochemistry 39(15):4309-4319 (2000).
de Beer, T., et al., Science 281(5381):1357-1360 (1998).
Chen, H., et al., Nature 394(6695):793-797 (1998).
Matsuda, M., et al., J. Biol. Chem. 271(24):14468-14472 (1996).
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