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SPPL2B Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SPPL2B Antibody (Center) AP21609c
    Anti-SPPL2B Antibody (Center)at 1:2000 dilution + human cerebellum lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8TCT7
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 64644 Da
Additional Information
Gene ID 56928
Other Names Signal peptide peptidase-like 2B, SPP-like 2B, SPPL2b, 3423-, Intramembrane protease 4, IMP-4, Presenilin homologous protein 4, PSH4, Presenilin-like protein 1, SPPL2B, IMP4, KIAA1532, PSL1
Target/Specificity This SPPL2B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 196-229 amino acids from the Central region of human SPPL2B.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPPL2B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPP2B
Function Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing (PubMed:16829952, PubMed:16829951, PubMed:17965014, PubMed:19114711, PubMed:22194595). Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus (PubMed:16829952, PubMed:16829951). May play a role in the regulation of innate and adaptive immunity (PubMed:16829952). Catalyzes the intramembrane cleavage of the simian foamy virus processed leader peptide gp18 of the envelope glycoprotein gp130 dependently of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis (PubMed:23132852).
Cellular Location Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein; Lumenal side Note=targeted through the entire secretory pathway to endosomes/lysosomes (PubMed:15998642)
Tissue Location Expressed predominantly in adrenal cortex and mammary gland.
Research Areas
Citations (0)

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Background

Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF- alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity.

References

Irmler M.,et al.Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
Martoglio B.,et al.Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
Grigorenko A.P.,et al.Biochemistry (Mosc.) 67:826-834(2002).
Nagase T.,et al.DNA Res. 7:143-150(2000).
Grimwood J.,et al.Nature 428:529-535(2004).

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$ 295.00
$ 99.00
Cat# AP21609c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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