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HGS Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | O14964 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 86192 Da |
| Antigen Region | 256-286 aa |
| Gene ID | 9146 |
|---|---|
| Other Names | Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, Protein pp110, HGS, HRS |
| Target/Specificity | This HGS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-286 amino acids from the N-terminal region of human HGS. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HGS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HGS |
|---|---|
| Synonyms | HRS |
| Function | Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin- coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation. |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q9JJ50}. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side Endosome, multivesicular body membrane {ECO:0000250|UniProtKB:Q9JJ50}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9JJ50} Note=Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments. |
| Tissue Location | Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes |
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Provided below are standard protocols that you may find useful for product applications.
Background
HGS is involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. It could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. HGS may concentrate ubiquitinated receptors within clathrin-coated regions. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM. This complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. HGS may contribute to the efficient recruitment of SMADs to the activin receptor complex.
References
Kirk E, et al. J Cell Sci. 2006 Nov 15;119(Pt 22):4689-701
Sasaki Y, et al. J Biol Chem. 2001 Aug 10;276(32):29943-52.
Miura S, et al. Mol Cell Biol. 2000 Dec;20(24):9346-55.
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