|Application ||WB, E|
|Calculated MW||25358 Da|
|Other Names||Proteasome subunit beta type-6, Macropain delta chain, Multicatalytic endopeptidase complex delta chain, Proteasome delta chain, Proteasome subunit Y, PSMB6, LMPY, Y|
|Target/Specificity||This PSMB6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 151-185 amino acids from the Central region of human PSMB6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PSMB6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB6 displays a peptidylglutamyl-hydrolizing activity also termed postacidic or caspase-like activity, meaning that the peptides bond hydrolysis occurs directly after acidic residues.|
|Cellular Location||Cytoplasm. Nucleus|
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Provided below are standard protocols that you may find useful for product applications.
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens.
Akiyama K.-Y.,et al.Science 265:1231-1234(1994).
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.
DeMartino G.N.,et al.Biochim. Biophys. Acta 1079:29-38(1991).
Lee L.W.,et al.Biochim. Biophys. Acta 1037:178-185(1990).
Lubec G.,et al.Submitted (MAR-2007) to UniProtKB.
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