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FEV Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • IHC-P - FEV Antibody (N-Term) AP21652a
    AP21652a staining FEV in human small intestine sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - FEV Antibody (N-Term) AP21652a
    Anti-FEV Antibody (N-Term) at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99581
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 25030 Da
Additional Information
Gene ID 54738
Other Names Protein FEV, Fifth Ewing variant protein, PC12 ETS domain-containing transcription factor 1, PC12 ETS factor 1, Pet-1, FEV, PET1
Target/Specificity This FEV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 17-49 amino acids from human FEV.
Dilution IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFEV Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FEV
Synonyms PET1
Function Functions as a transcriptional regulator. According to PubMed:12761502, it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00237, ECO:0000269|PubMed:12761502}
Tissue Location In brain, exclusively expressed in the major serotonergic neurons of the dorsal and median raphe nuclei located in the midbrain and pons. Also detected in prostate and small intestine.
Research Areas
Citations (0)

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Functions as a transcriptional regulator. According to PubMed:12761502, it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth.


Peter M.,et al.Oncogene 14:1159-1164(1997).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Maurer P.,et al.Oncogene 22:3319-3329(2003).
Maurer P.,et al.Neurosci. Lett. 357:215-218(2004).

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$ 295.00
$ 99.00
Cat# AP21652a
(40 western blots)
Availability: In Stock
Bulk Size
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