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>   home   >   Products   >   Primary Antibodies   >   Cardiovascular   >   TFCP2 Antibody (Center)   

TFCP2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TFCP2 Antibody (Center) AP21657c
    Anti-TFCP2 Antibody (Center) at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q12800
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 57256 Da
Additional Information
Gene ID 7024
Other Names Alpha-globin transcription factor CP2, SAA3 enhancer factor, Transcription factor LSF, TFCP2, LSF, SEF
Target/Specificity This TFCP2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 282-316 amino acids from the Central region of human TFCP2.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTFCP2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TFCP2
Synonyms LSF, SEF
Function Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 (By similarity). Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter.
Cellular Location Nucleus
Tissue Location Ubiquitous. Expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung (at protein level)
Research Areas
Citations (0)

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Background

Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 (By similarity). Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter.

References

Lim L.C.,et al.Mol. Cell. Biol. 12:828-835(1992).
Yoon J.-B.,et al.Mol. Cell. Biol. 14:1776-1785(1994).
Shirra M.K.,et al.Mol. Cell. Biol. 14:5076-5087(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP21657c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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