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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   UBCH9 Antibody (N-term)   

UBCH9 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - UBCH9 Antibody (N-term) AP2170a
    Western blot analysis of anti-UBCH9 Antibody (N-term) (Cat.#AP2170a) in Y79 cell line lysates (35ug/lane). UBCH9 (arrow) was detected using the purified Pab.
  • IHC-P - UBCH9 Antibody (N-term) AP2170a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q969T4
Other Accession P52483, Q2T9X7
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22913 Da
Antigen Region 10-40 aa
Additional Information
Gene ID 10477
Other Names Ubiquitin-conjugating enzyme E2 E3, UbcH9, Ubiquitin carrier protein E3, Ubiquitin-conjugating enzyme E2-23 kDa, Ubiquitin-protein ligase E3, UBE2E3, UBCE4, UBCH9
Target/Specificity This UBCH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-40 amino acids from the N-terminal region of human UBCH9.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBCH9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2E3
Synonyms UBCE4, UBCH9
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.
Cellular Location Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner
Tissue Location Ubiquitously expressed at low levels. Highly expressed in skeletal muscle.
Research Areas
Citations (0)

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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBCH9 is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found.


Ito, K., et al., Cytogenet. Cell Genet. 84 (1-2), 99-104 (1999).

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$ 295.00
$ 99.00
Cat# AP2170a
(40 western blots)
Availability: In Stock
Bulk Size
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