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ITCH/AIP4 Antibody (C-Term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP2171b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ITCH/AIP4 Antibody (C-Term) - Product info | |
| Application | WB
|
| Primary Accession | Q96J02 |
| Other Accession | Q8C863, Q9BY75 |
| Reactivity | Human |
| Predicted | Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 102803 Da |
ITCH/AIP4 Antibody (C-Term) - Additional info | |
| Gene ID 83737 | |
| Other Names ITCH; E3 ubiquitin-protein ligase Itchy homolog; Atrophin-1-interacting protein 4; NFE2-associated polypeptide 1 | |
| Target/Specificity This ITCH/AIP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 772~801 amino acids from the C-terminal region of human ITCH. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ITCH/AIP4 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ITCH/AIP4 Antibody (C-Term) - Protein Information | |
| Name ITCH | |
| Function Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1 Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Critical regulator of T-helper (TH2) cytokine development through its ability to induce JUNB ubiquitination and degradation (By similarity). Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It is involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination | |
| Cellular Location Cell membrane. Cytoplasm (By similarity). Nucleus. Note=Associates with endocytic vesicles. May be recruited to exosomes by NDFIP1 | |
| Tissue Location Widely expressed. | |
ITCH/AIP4 Antibody (C-Term) - Related products
AP2171a: ITCH/AIP4 Antibody (N-Term)
AP2171b: ITCH/AIP4 Antibody (C-Term)
BP2171a: ITCH Antibody (N-term) Blocking Peptide
ITCH/AIP4 Antibody (C-Term) - Application data
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The anti-ITCH Pab (Cat. #AP2171b) is used in Western blot to detect ITCH in HL-60 cell lysate.
ITCH/AIP4 Antibody (C-Term) - Research Areas
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BACKGROUND
ITCH is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and has been shown to interact via its WW domains with DRPLA, NFE2 and CBLC. Other potential interaction partners include NOTCH1, OCLN, JUN and JUNB .
REFERENCES
Courbard, J.R., et al., J. Biol. Chem. 277(47):45267-45275 (2002). Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Deloukas, P., et al., Nature 414(6866):865-871 (2001). Chen, X., et al., Genomics 73(2):238-241 (2001). Winberg, G., et al., Mol. Cell. Biol. 20(22):8526-8535 (2000).
