SAG Antibody (C-Term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | P10523 |
Reactivity | Mouse |
Host | Rabbit |
Clonality | polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 45120 Da |
Antigen Region | 273-307 aa |
Gene ID | 6295 |
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Other Names | S-arrestin, 48 kDa protein, Retinal S-antigen, S-AG, Rod photoreceptor arrestin, SAG |
Target/Specificity | This SAG antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 273-307 amino acids from the human SAG. |
Dilution | WB~~1:2000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SAG Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SAG |
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Function | Binds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO (By similarity). May play a role in preventing light-dependent degeneration of retinal photoreceptor cells (PubMed:9565049). |
Cellular Location | Cell projection, cilium, photoreceptor outer segment. Membrane {ECO:0000250|UniProtKB:P20443}; Peripheral membrane protein {ECO:0000250|UniProtKB:P20443}. Note=Highly expressed in photoreceptor outer segments in light-exposed retina. Evenly distributed throughout rod photoreceptor cells in dark-adapted retina (By similarity) Predominantly dectected at the proximal region of photoreceptor outer segments, near disk membranes (PubMed:3720866) {ECO:0000250|UniProtKB:P08168, ECO:0000269|PubMed:3720866} |
Tissue Location | Detected in retina, in the proximal portion of the outer segment of rod photoreceptor cells (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated- phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase.
References
Yamaki K.,et al.FEBS Lett. 234:39-43(1988).
Yamaki K.,et al.FEBS Lett. 236:507-507(1988).
Yamamoto S.,et al.Nat. Genet. 15:175-178(1997).
Hillier L.W.,et al.Nature 434:724-731(2005).
Roni V.,et al.BMC Genomics 8:42-42(2007).
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