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Mib1/Mindbomb Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Mib1/Mindbomb Antibody (N-term) AP2172a
    MIB Antibody (K28) (Cat. #AP2172a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MIB antibody detected the MIB protein (arrow).
  • IF - Mib1/Mindbomb Antibody (N-term) AP2172a
    Immunofluorescent staining of Hela cells incubated with MIB Antibody (N-term) (Cat # AP2172a) at a dilution of 1:20. Data courtesy of Dr. Vyacheslav Akimov, University of Southern Denmark.
  • IHC-P - Mib1/Mindbomb Antibody (N-term) AP2172a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P, E
Primary Accession Q86YT6
Other Accession Q6GNY1, Q80SY4, Q804S5
Reactivity Human
Predicted Zebrafish, Mouse, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 110136 Da
Antigen Region 13-42 aa
Additional Information
Gene ID 57534
Other Names E3 ubiquitin-protein ligase MIB1, 632-, DAPK-interacting protein 1, DIP-1, Mind bomb homolog 1, Zinc finger ZZ type with ankyrin repeat domain protein 2, MIB1, DIP1, KIAA1323, ZZANK2
Target/Specificity This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Mib1/Mindbomb.
Dilution WB~~1:1000
IF~~1:20~100
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMib1/Mindbomb Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MIB1
Synonyms DIP1, KIAA1323, ZZANK2
Function E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite Cell membrane. Note=Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear Displaced from centriolar satellites in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock
Tissue Location Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
Research Areas
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Background

MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.

References

Itoh, M., et al., Dev. Cell 4(1):67-82 (2003).
Jin, Y., et al., J. Biol. Chem. 277(49):46980-46986 (2002).

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Cat# AP2172a
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