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NDUFB3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - NDUFB3 Antibody (Center) AP21738c
    All lanes : Anti-NDUFB3 Antibody (Center) at 1:2000 dilution Lane 1: human heart lysate Lane 2: mouse heart lysate Lane 3: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O43676
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 11402 Da
Additional Information
Gene ID 4709
Other Names NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3, Complex I-B12, CI-B12, NADH-ubiquinone oxidoreductase B12 subunit, NDUFB3
Target/Specificity This NDUFB3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 14-49 amino acids from the Central region of human NDUFB3.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFB3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFB3
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein; Matrix side
Research Areas
Citations (0)

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Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

References

Ton C.,et al.Biochem. Biophys. Res. Commun. 241:589-594(1997).
Loeffen J.L.C.M.,et al.Biochem. Biophys. Res. Commun. 253:415-422(1998).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP21738c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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