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>   home   >   Products   >   Primary Antibodies   >   RAD23A Antibody (C-term)   

RAD23A Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RAD23A Antibody (C-term) AP2173b
    Western blot analysis of RAD23A Antibody (C-term) (Cat.#AP2173b) in HL60 cell line lysates (35ug/lane). RAD23A (arrow) was detected using the purified Pab.
  • IHC-P - RAD23A Antibody (C-term) AP2173b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54725
Other Accession Q4KMA2, P54728, P54727, Q29RK4, P54726, A3KMV2, NP_005044
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39609 Da
Antigen Region 303-333 aa
Additional Information
Gene ID 5886
Other Names UV excision repair protein RAD23 homolog A, HR23A, hHR23A, RAD23A
Target/Specificity This RAD23A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-333 amino acids from the C-terminal region of human RAD23A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAD23A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD23A
Function Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. Involved in vpr-dependent replication of HIV-1 in non- proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome.
Cellular Location Nucleus.
Citations (0)

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RAD223A is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.


Mueller, T.D., et al., EMBO J. 22(18):4634-4645 (2003).
Mueller, T.D., et al., J. Mol. Biol. 319(5):1243-1255 (2002).
Elder, R.T., et al., Nucleic Acids Res. 30(2):581-591 (2002).
Chen, L., et al., EMBO Rep. 2(10):933-938 (2001).
Gaynor, E.M., et al., Exp. Cell Res. 267(2):243-257 (2001).

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$ 295.00
$ 99.00
Cat# AP2173b
(40 western blots)
Availability: In Stock
Bulk Size
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