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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   WDR51B Antibody (C-term)   

WDR51B Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - WDR51B Antibody (C-term) AP21756b
    All lanes : Anti-WDR51B Antibody (C-term) at 1:2000 dilution Lane 1: human testis lysate Lane 2: MDA-MB-453 whole cell lysate Lane 3: TT whole cell lysate Lane 4: Y79 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8TC44
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 53668 Da
Additional Information
Gene ID 282809
Other Names POC1 centriolar protein homolog B, Pix1, Proteome of centriole protein 1B, WD repeat-containing protein 51B, POC1B, WDR51B
Target/Specificity This WDR51B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 386-417 amino acids from the C-terminal region of human WDR51B.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWDR51B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POC1B
Synonyms WDR51B
Function Plays an important role in centriole assembly and/or stability and ciliogenesis (PubMed:20008567). Involved in early steps of centriole duplication, as well as in the later steps of centriole length control (PubMed:19109428). Acts in concert with POC1A to ensure centriole integrity and proper mitotic spindle formation. Required for primary cilia formation, ciliary length and also cell proliferation (PubMed:23015594). Required for retinal integrity (PubMed:25044745).
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, spindle pole. Note=Component of both mother and daughter centrioles Localizes to the basal body and centriole adjacent to the connecting cilium of photoreceptors and in synapses of the outer plexiform layer. {ECO:0000250|UniProtKB:Q8BHD1}
Tissue Location Expressed in the retina.
Research Areas
Citations (0)

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Background

Plays an important role in centriole assembly and/or stability and ciliogenesis (PubMed:20008567). Involved in early steps of centriole duplication, as well as in the later steps of centriole length control (PubMed:19109428). Acts in concert with POC1A to ensure centriole integrity and proper mitotic spindle formation. Required for primary cilia formation, ciliary length and also cell proliferation (PubMed:23015594). Required for retinal integrity (PubMed:25044745).

References

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.E.,et al.Nature 440:346-351(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Hames R.S.,et al.Exp. Cell Res. 314:574-589(2008).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

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$ 99.00
Cat# AP21756b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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