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ERVK-7 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - ERVK-7 Antibody (N-Term) AP21832a
    AP21832a staining ERVK-7 in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - ERVK-7 Antibody (N-Term) AP21832a
    AP21832a staining ERVK-7 in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - ERVK-7 Antibody (N-Term) AP21832a
    All lanes : Anti-ERVK-7 Antibody (N-Term) at 1:1000-1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: human lung lysate Lane 3: human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P61567
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 66649 Da
Additional Information
Other Names Endogenous retrovirus group K member 7 Env polyprotein, Envelope polyprotein, HERV-K(III) envelope protein, HERV-K102 envelope protein, HERV-K_1q22 provirus ancestral Env polyprotein, Surface protein, SU, Transmembrane protein, TM, ERVK-7
Target/Specificity This ERVK-7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 96-128 amino acids from human ERVK-7.
Dilution IHC-P~~1:25
WB~~1:1000-1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsERVK-7 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ERVK-7
Function Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane (By similarity).
Cellular Location Virion.
Tissue Location Expressed in lung, placenta, testis and peripheral blood lymphocytes.
Research Areas
Citations (0)

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Background

Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane (By similarity).

References

Barbulescu M.,et al.Curr. Biol. 9:861-868(1999).
Sugimoto J.,et al.Genomics 72:137-144(2001).
Wang-Johanning F.,et al.Oncogene 22:1528-1535(2003).

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$ 295.00
$ 99.00
Cat# AP21832a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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