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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   NDC80 Antibody (C-Term)   

NDC80 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - NDC80 Antibody (C-Term) AP21833b
    All lanes : Anti-NDC80 Antibody (C-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: K562 whole cell lysate Lane 5: 293T/17 whole cell lysate Lane 6: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O14777
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 73913 Da
Additional Information
Gene ID 10403
Other Names Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, NDC80, HEC, HEC1, KNTC2
Target/Specificity This NDC80 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 594-628 amino acids from human NDC80.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDC80 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDC80
Synonyms HEC, HEC1, KNTC2
Function Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Cellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore
Research Areas
Citations (0)

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Background

Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

References

Chen Y.,et al.Mol. Cell. Biol. 17:6049-6056(1997).
Chen Y.,et al.J. Biol. Chem. 272:24081-24087(1997).
Zheng L.,et al.Mol. Cell. Biol. 19:5417-5428(1999).
Zheng L.,et al.Mol. Cell. Biol. 20:3529-3537(2000).
Chen Y.,et al.J. Biol. Chem. 277:49408-49416(2002).

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$ 295.00
$ 99.00
Cat# AP21833b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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