|Application ||WB, E|
|Calculated MW||73913 Da|
|Other Names||Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, NDC80, HEC, HEC1, KNTC2|
|Target/Specificity||This NDC80 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 594-628 amino acids from human NDC80.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NDC80 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HEC, HEC1, KNTC2|
|Function||Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Chen Y.,et al.Mol. Cell. Biol. 17:6049-6056(1997).
Chen Y.,et al.J. Biol. Chem. 272:24081-24087(1997).
Zheng L.,et al.Mol. Cell. Biol. 19:5417-5428(1999).
Zheng L.,et al.Mol. Cell. Biol. 20:3529-3537(2000).
Chen Y.,et al.J. Biol. Chem. 277:49408-49416(2002).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.