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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GLS Antibody (C-Term)   

GLS Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - GLS Antibody (C-Term) AP21834b
    All lanes : Anti-GLS Antibody (C-Term) at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O94925
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 73461 Da
Additional Information
Gene ID 2744
Other Names Glutaminase kidney isoform, mitochondrial, GLS, K-glutaminase, L-glutamine amidohydrolase, GLS, GLS1, KIAA0838
Target/Specificity This GLS antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 555-587 amino acids from human GLS.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGLS Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GLS
Synonyms GLS1, KIAA0838
Function Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
Cellular Location Isoform 1: Cytoplasm, cytosol.
Tissue Location Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver Isoform 2 is expressed in cardiac and skeletal muscle
Research Areas
Citations (0)

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Background

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.

References

Elgadi K.M.,et al.Physiol. Genomics 1:51-62(1999).
Nagase T.,et al.DNA Res. 5:355-364(1998).
Chavez R.A.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Holcomb T.,et al.Brain Res. Mol. Brain Res. 76:56-63(2000).
Turner A.,et al.Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP21834b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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