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MYO5B Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MYO5B Antibody (N-Term) AP21849a
    Anti-MYO5B Antibody (N-Term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 214 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9ULV0
Other Accession P21271, P70569
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 213672 Da
Additional Information
Gene ID 4645
Other Names Unconventional myosin-Vb, MYO5B, KIAA1119
Target/Specificity This MYO5B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 7-30 amino acids from MYO5B.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMYO5B Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYO5B
Synonyms KIAA1119
Function May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P70569}.
Research Areas
Citations (0)

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Background

May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis.

References

Hirosawa M.,et al.DNA Res. 6:329-336(1999).
Ohara O.,et al.Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Yamakawa H.,et al.Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
Nusbaum C.,et al.Nature 437:551-555(2005).

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$ 295.00
$ 99.00
Cat# AP21849a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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