|Application ||WB, E|
|Calculated MW||232191 Da|
|Other Names||Fanconi anemia group M protein, Protein FACM, ATP-dependent RNA helicase FANCM, Fanconi anemia-associated polypeptide of 250 kDa, FAAP250, Protein Hef ortholog, FANCM, KIAA1596|
|Target/Specificity||This FANCM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1752-1783 amino acids from human FANCM.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FANCM Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA-dependent ATPase component of the Fanconi anemia (FA) core complex (PubMed:16116422). Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage (PubMed:16116422, PubMed:19423727, PubMed:20347428, PubMed:20347429). In complex with CENPS and CENPX, binds double-stranded DNA (dsDNA), fork-structured DNA (fsDNA) and Holliday junction substrates (PubMed:20347428, PubMed:20347429). Its ATP-dependent DNA branch migration activity can process branched DNA structures such as a movable replication fork. This activity is strongly stimulated in the presence of CENPS and CENPX (PubMed:20347429). In complex with FAAP24, efficiently binds to single-strand DNA (ssDNA), splayed-arm DNA, and 3'-flap substrates (PubMed:17289582). In vitro, on its own, strongly binds ssDNA oligomers and weakly fsDNA, but does not bind to dsDNA (PubMed:16116434).|
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Provided below are standard protocols that you may find useful for product applications.
ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. Binds to ssDNA but not to dsDNA. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions.
Meetei A.R.,et al.Nat. Genet. 37:958-963(2005).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Heilig R.,et al.Nature 421:601-607(2003).
Nagase T.,et al.DNA Res. 7:273-281(2000).
Mosedale G.,et al.Nat. Struct. Mol. Biol. 12:763-771(2005).
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