|Application ||WB, IHC-P, FC, E|
|Calculated MW||19458 Da|
|Other Names||Ubiquitin-fold modifier-conjugating enzyme 1, Ufm1-conjugating enzyme 1, UFC1|
|Target/Specificity||This Ufc1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Ufc1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Ufc1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.|
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Provided below are standard protocols that you may find useful for product applications.
Various ubiquitin-like (UBL) post-translational modifiers contribute critically to the protein degradation pathway . These UBLs are covalently linked to most target proteins through an enzymatic cascade analogous to ubiquitylation, consisting of E1 (activating), E2 (conjugating), and E3 (ligating) enzymes. Ufc1 Belongs to the ubiquitin-conjugating enzyme family, and is an E2-like enzyme which forms an intermediate with UFM1, a novel ubiquitin-fold modifier, via thioester linkage.
Komatsu, M., et al., EMBO J. 23(9):1977-1986 (2004).
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