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GNG2 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - GNG2 Antibody (N-Term) AP21903a
    AP21903a staining GNG2 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - GNG2 Antibody (N-Term) AP21903a
    All lanes : Anti-GNG2 Antibody (N-Term) at 1:8000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 8 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P59768
Other Accession P63212, P63213, Q5R7U4
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 7850 Da
Additional Information
Gene ID 54331
Other Names Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, G gamma-I, GNG2
Target/Specificity This GNG2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-52 amino acids from of human GNG2.
Dilution IHC-P~~1:25
WB~~1:8000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNG2 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNG2
Function Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction (By similarity).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side
Tissue Location Expressed in fetal tissues, including testis, adrenal gland, brain, white blood cells and brain
Research Areas
Citations (0)

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Background

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction (By similarity).

References

Modarressi M.H.,et al.Biochem. Biophys. Res. Commun. 272:610-615(2000).
Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).

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$ 295.00
$ 99.00
Cat# AP21903a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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