|Application ||WB, E|
|Calculated MW||161813 Da|
|Other Names||Collagen alpha-3(IV) chain, Goodpasture antigen, Tumstatin, COL4A3|
|Target/Specificity||This COL4A3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 141-173 amino acids from human COL4A3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||COL4A3 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.|
|Cellular Location||Secreted, extracellular space, extracellular matrix, basement membrane. Note=Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina (BL).|
|Tissue Location||Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. According to PubMed:8083201, alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. According to PubMed:7523402, alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells|
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Provided below are standard protocols that you may find useful for product applications.
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Mariyama M.,et al.J. Biol. Chem. 269:23013-23017(1994).
Leinonen A.,et al.Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
Heidet L.,et al.J. Am. Soc. Nephrol. 12:97-106(2001).
Hillier L.W.,et al.Nature 434:724-731(2005).
Momota R.,et al.FEBS Lett. 424:11-16(1998).
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